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ic21 murine peritoneal macrophage cell line (ATCC)

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ATCC ic21 murine peritoneal macrophage cell line

Pten -deficient ovarian cancer cells alter their tumor immune microenvironment towards a suppressive state via polarizing macrophages to M2-like phenotype. Total RNA from untreated tumors of different genotypes were subjected to NanoString gene expression profiling using the PanCancer immune gene panel displayed as a (A) volcano plot showing differential expression pattern of genes associated with <t>macrophage</t> function. (B) Proportion of cells derived from ascites of mice injected with ID8- Trp53 −/− ; Pten −/− and ID8- Trp53 −/− ; Brca1 −/− cells expressing CD45+CD11b+F4/80+cells. (C) MCP-1 chemokine levels derived from the ascites fluid of untreated mice injected with ID8- Trp53 −/− ; Pten −/− and ID8- Trp53 −/− ; Brca1 −/− cells. (D) Transwell migration assay of <t>IC21</t> <t>peritoneal</t> macrophage cells stimulated (from left to right) with media control (Dulbecco’s Modified Eagle’s Medium), positive control (20 ng/mL MCP-1), or 72 hours conditioned media of ID8- Trp53 −/− ; Pten −/− and ID8- Trp53 −/− ; Brca1 −/− cells. Stimulated for 16 hours; images 20x magnification. Flow cytometric analysis of IC21 macrophage proportions expressing (E) CD45+F4/80+CD206+ post-stimulation with concentrated ascites derived from mice injected with different genotypes of HGSC cells. US, unstimulated IC21 cells; M1 positive control represented by IC21 cells stimulated with IFN-ɣ (50 ng/mL) and LPS (100 ng/mL) in complete media; M2 positive control represented by IC21 cells stimulated with IL-4 (10 ng/mL) and IL-10 (20 ng/mL) in complete media. Averages of triplicate wells/experiment for three repeated experiments displayed. Mann-Whitney non-parametric test was used for (B–C) *p<0.05 **p<0.005, ***p<0.001, ****p<0.0001. One-way analysis of variance applied for D and E. Mean±SD. HGSC, high-grade serous ovarian cancer; IFN, interferon; IL, interleukin; MCP-1, macrophage chemoattractant protein 1; ns, not significant; PTEN, phosphatase and tensin homolog.

Ic21 Murine Peritoneal Macrophage Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/murine+macrophages+cell+line+ic21/pmc10083863-45-0-9?v=ATCC
Average 95 stars, based on 255 article reviews

ic21 murine peritoneal macrophage cell line - by Bioz Stars, 2026-08

95/100 stars

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1) Product Images from "Cancer cell genotype associated tumor immune microenvironment exhibits differential response to therapeutic STING pathway activation in high-grade serous ovarian cancer"

Article Title: Cancer cell genotype associated tumor immune microenvironment exhibits differential response to therapeutic STING pathway activation in high-grade serous ovarian cancer

Journal: Journal for Immunotherapy of Cancer

doi: 10.1136/jitc-2022-006170

Figure Legend Snippet: Pten -deficient ovarian cancer cells alter their tumor immune microenvironment towards a suppressive state via polarizing macrophages to M2-like phenotype. Total RNA from untreated tumors of different genotypes were subjected to NanoString gene expression profiling using the PanCancer immune gene panel displayed as a (A) volcano plot showing differential expression pattern of genes associated with macrophage function. (B) Proportion of cells derived from ascites of mice injected with ID8- Trp53 −/− ; Pten −/− and ID8- Trp53 −/− ; Brca1 −/− cells expressing CD45+CD11b+F4/80+cells. (C) MCP-1 chemokine levels derived from the ascites fluid of untreated mice injected with ID8- Trp53 −/− ; Pten −/− and ID8- Trp53 −/− ; Brca1 −/− cells. (D) Transwell migration assay of IC21 peritoneal macrophage cells stimulated (from left to right) with media control (Dulbecco’s Modified Eagle’s Medium), positive control (20 ng/mL MCP-1), or 72 hours conditioned media of ID8- Trp53 −/− ; Pten −/− and ID8- Trp53 −/− ; Brca1 −/− cells. Stimulated for 16 hours; images 20x magnification. Flow cytometric analysis of IC21 macrophage proportions expressing (E) CD45+F4/80+CD206+ post-stimulation with concentrated ascites derived from mice injected with different genotypes of HGSC cells. US, unstimulated IC21 cells; M1 positive control represented by IC21 cells stimulated with IFN-ɣ (50 ng/mL) and LPS (100 ng/mL) in complete media; M2 positive control represented by IC21 cells stimulated with IL-4 (10 ng/mL) and IL-10 (20 ng/mL) in complete media. Averages of triplicate wells/experiment for three repeated experiments displayed. Mann-Whitney non-parametric test was used for (B–C) *p<0.05 **p<0.005, ***p<0.001, ****p<0.0001. One-way analysis of variance applied for D and E. Mean±SD. HGSC, high-grade serous ovarian cancer; IFN, interferon; IL, interleukin; MCP-1, macrophage chemoattractant protein 1; ns, not significant; PTEN, phosphatase and tensin homolog.

Techniques Used: Gene Expression, Quantitative Proteomics, Derivative Assay, Injection, Expressing, Transwell Migration Assay, Control, Modification, Positive Control, MANN-WHITNEY

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Journal: Journal for Immunotherapy of Cancer

Article Title: Cancer cell genotype associated tumor immune microenvironment exhibits differential response to therapeutic STING pathway activation in high-grade serous ovarian cancer

doi: 10.1136/jitc-2022-006170

Figure Lengend Snippet: Pten -deficient ovarian cancer cells alter their tumor immune microenvironment towards a suppressive state via polarizing macrophages to M2-like phenotype. Total RNA from untreated tumors of different genotypes were subjected to NanoString gene expression profiling using the PanCancer immune gene panel displayed as a (A) volcano plot showing differential expression pattern of genes associated with macrophage function. (B) Proportion of cells derived from ascites of mice injected with ID8- Trp53 −/− ; Pten −/− and ID8- Trp53 −/− ; Brca1 −/− cells expressing CD45+CD11b+F4/80+cells. (C) MCP-1 chemokine levels derived from the ascites fluid of untreated mice injected with ID8- Trp53 −/− ; Pten −/− and ID8- Trp53 −/− ; Brca1 −/− cells. (D) Transwell migration assay of IC21 peritoneal macrophage cells stimulated (from left to right) with media control (Dulbecco’s Modified Eagle’s Medium), positive control (20 ng/mL MCP-1), or 72 hours conditioned media of ID8- Trp53 −/− ; Pten −/− and ID8- Trp53 −/− ; Brca1 −/− cells. Stimulated for 16 hours; images 20x magnification. Flow cytometric analysis of IC21 macrophage proportions expressing (E) CD45+F4/80+CD206+ post-stimulation with concentrated ascites derived from mice injected with different genotypes of HGSC cells. US, unstimulated IC21 cells; M1 positive control represented by IC21 cells stimulated with IFN-ɣ (50 ng/mL) and LPS (100 ng/mL) in complete media; M2 positive control represented by IC21 cells stimulated with IL-4 (10 ng/mL) and IL-10 (20 ng/mL) in complete media. Averages of triplicate wells/experiment for three repeated experiments displayed. Mann-Whitney non-parametric test was used for (B–C) *p<0.05 **p<0.005, ***p<0.001, ****p<0.0001. One-way analysis of variance applied for D and E. Mean±SD. HGSC, high-grade serous ovarian cancer; IFN, interferon; IL, interleukin; MCP-1, macrophage chemoattractant protein 1; ns, not significant; PTEN, phosphatase and tensin homolog.

Article Snippet: IC21 murine peritoneal macrophage cell line was obtained from American Type Culture Collection (Manassas, Virginia, USA) and cultured in RPMI 1640 (Sigma-Aldrich, Ontario, Canada) supplemented with 10% FBS and 1% penicillin/streptomycin.

Techniques: Gene Expression, Quantitative Proteomics, Derivative Assay, Injection, Expressing, Transwell Migration Assay, Control, Modification, Positive Control, MANN-WHITNEY

IL-36γ is secreted in response to ATP in a non-conventional pathway. (A) IL-36γ protein expression in lysates (L) and supernatants (S) of LPS or LPS/ATP activated macrophages. Densitometry of IL-36γ intensity in lysates (B) and supernatants (C) of cell stimulated with LPS or LPS/ATP (L/A). (D) IF of IC21 macrophages stimulated with LPS/ATP (L/A) and treated with monensin (Mon) or brefeldin A (BfA). *p<0.05, **p<0.01, ***p<0.001.

Journal: Frontiers in Immunology

Article Title: IL-36γ is secreted through an unconventional pathway using the Gasdermin D and P2X7R membrane pores

doi: 10.3389/fimmu.2022.979749

Figure Lengend Snippet: IL-36γ is secreted in response to ATP in a non-conventional pathway. (A) IL-36γ protein expression in lysates (L) and supernatants (S) of LPS or LPS/ATP activated macrophages. Densitometry of IL-36γ intensity in lysates (B) and supernatants (C) of cell stimulated with LPS or LPS/ATP (L/A). (D) IF of IC21 macrophages stimulated with LPS/ATP (L/A) and treated with monensin (Mon) or brefeldin A (BfA). *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: Murine macrophages cell line IC21 (TIB-186) was cultured according to the manufacturer’s instructions (ATCC, VA).

Techniques: Expressing

IL-36γ is localized in the cytoplasm of IC21 macrophages. IL-36γ localization in IC21 macrophages in control, LPS, LPS/ATP (L/A), LPS/ATP+A438, and LPS/ATP+NSA conditions. nuclei staining (DAPI), IL-36γ (Alexa Fluor594).